Validation of the growth hormone (GH)-dependent marker method of detecting GH abuse in sport through the use of independent data sets
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Growth Horm IGF Res. 2007 Oct;17(5):416-23. Epub 2007 Jun 20.
Erotokritou-Mulligan I, Bassett EE, Kniess A, Sonksen PH, Holt RI.
Endocrinology and Metabolism Sub-Division, School of Medicine, University of Southampton, IDS Building (MP887), Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom.
CONTEXT: The detection of exogenously administered growth hormone (GH) poses a formidable challenge but a detection method based on the measurement of two GH-dependent markers, IGF-I and type 3 pro-collagen (P-III-P) has been proposed. The measurement of multiple markers in conjunction with discriminant functions can improve the sensitivity and specificity of detection compared with single marker analysis.
OBJECTIVE: To provide further validation of the GH-dependent marker approach.
DESIGN: Analysis of discriminant function scores for GH detection on independent datasets.
SETTING: Two independent (GH-2000 and Kreischa) double blind, placebo controlled, hGH administration studies. SUBJECTS: Healthy active male volunteers.
INTERVENTION: GH-2000 proposed a discriminant function involving IGF-I and P-III- P while the Kreischa function involved IGF-I, P-III-P and IGFBP-3. After adjustment for assay differences the formulae were applied to the other dataset.
OUTCOME MEASURES: Ability to detect GH use in independent datasets using a predefined specificity of approximately 1 in 10000. RESULTS: The GH-2000 formula was able to detect 90% of those receiving GH in the Kreischa study at one or more time points during the study period. This sensitivity was similar to that obtained on the original GH-2000 dataset. The Kreischa formula correctly identified 41% of individuals receiving GH in the GH-2000 study.
CONCLUSIONS: The study provides further validation that the test proposed by GH-2000 based on IGF-I and P-III-P concentrations can be used to detect subjects receiving exogenous GH.
PubMed ID and Record
Growth Horm IGF Res. 2007 Oct;17(5):416-23. Epub 2007 Jun 20.
Erotokritou-Mulligan I, Bassett EE, Kniess A, Sonksen PH, Holt RI.
Endocrinology and Metabolism Sub-Division, School of Medicine, University of Southampton, IDS Building (MP887), Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom.
CONTEXT: The detection of exogenously administered growth hormone (GH) poses a formidable challenge but a detection method based on the measurement of two GH-dependent markers, IGF-I and type 3 pro-collagen (P-III-P) has been proposed. The measurement of multiple markers in conjunction with discriminant functions can improve the sensitivity and specificity of detection compared with single marker analysis.
OBJECTIVE: To provide further validation of the GH-dependent marker approach.
DESIGN: Analysis of discriminant function scores for GH detection on independent datasets.
SETTING: Two independent (GH-2000 and Kreischa) double blind, placebo controlled, hGH administration studies. SUBJECTS: Healthy active male volunteers.
INTERVENTION: GH-2000 proposed a discriminant function involving IGF-I and P-III- P while the Kreischa function involved IGF-I, P-III-P and IGFBP-3. After adjustment for assay differences the formulae were applied to the other dataset.
OUTCOME MEASURES: Ability to detect GH use in independent datasets using a predefined specificity of approximately 1 in 10000. RESULTS: The GH-2000 formula was able to detect 90% of those receiving GH in the Kreischa study at one or more time points during the study period. This sensitivity was similar to that obtained on the original GH-2000 dataset. The Kreischa formula correctly identified 41% of individuals receiving GH in the GH-2000 study.
CONCLUSIONS: The study provides further validation that the test proposed by GH-2000 based on IGF-I and P-III-P concentrations can be used to detect subjects receiving exogenous GH.
PubMed ID and Record
posted by Dr.Koudinov @ 12:24 PM
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